Pan-neuronal imaging
Green intensity is a proxy for neuronal activity. Movie is 10x real time.
We constructed a C. elegans strain that expresses GFP/RFP in all neurons and utilize pan-neuronal calcium imaging to broadly screen the activity of its nervous system in response to thermal stimuli. [REF]
Tracking C. elegans
Left side shows C. elegans movements. Right side shows the position on a 9 cm agar plate (red square).
We use tracking data to model locomotion and behavior of C. elegans in response to sensory information. [REF]
Bacterial colony interface
E. coli colonies grown from single cells. Bacteria are identical except for one strain is expressing Green Fluorescent Protein (GFP) and the other strain is expressing Red Fluorescent Protein so that the interface between colonies can be measured as a function of various conditions.
Tracking guppy pairs
The social behavior of guppies was studied using this tank where they were tracked in 3D. For the guppy pair (dyad) from the high-predation population, the closer the individuals were to each other, the more likely they were to be parallel, but no correlation was found for the low-predation P. reticulata. The startle response of P. reticulata comprised the following sequence: freezing, darting and skittering and recovery to pre-stimulus swimming behaviour. Upon repeated encounters with the stimulus, a reduced shoaling and startle response was observed, although the rate of reduction was faster in P. reticulata from the high-predation population than those from the low-predation population. [REF]
C. elegans thermotaxis
C. elegans will crawl toward their thermal preference when placed on a thermal gradient. The movies below show worms placed on a thermal gradient where the left side is cold and the right side is warm. The left movie shows worms performing thermotaxis to a temperature on the right side of the plate. The right movie shows worm performing isothermal tracking where worms are placed at their thermal preference (center) and so they follow isothermal lines which are vertically oriented on the plate.
E. coli: swimming and tethered
Phase microscopy video of swimming bacteria (left) and cells tethered to the microscope slide by one of their rotary motors.
